ABSTRACT
Objective: To discover the mechanism behind ameliorative effects of Michelia champaca (M. champaca) in gastrointestinal, respiratory and cardiovascular disorders. Methods: Anti- spasmodic potential was evaluated by trying the M. champaca extract (aqueous:ethanolic) on rabbit aorta, trachea and jejunum in vitro. Isotonic and isometric transducers coupled with Power Lab data acquisition system was used to record the responses of isolated tissues. Results: M. champaca extract relaxed the spontaneous and high K
ABSTRACT
In vivo and in vitro research study was conducted on Cyperus rotundus to evaluate the sound mechanistic background in the treatment of gastrointestinal, bronchial and vascular disorders as well as in pain, emesis, pyrexia and bacterial infections. Results showed that crude extract of Cyperus rotundus [Cr.Cr] exhibited the dose-dependent spasmolytic effect in rabbit jejunum by inhibiting the spontaneous and K+ [80 mM]-induced contractions. Pretreatment of tissue with Cr. Cr caused the rightward shift of calcium concentration response curves, similar to verapamil. Cr. Cr also caused the relaxation of K+ [80 mM]- and carbachol [1 microM]-induced contractions of trachea preparations, similar to that of verapamil. Moreover, Cr. Cr also relaxed the contraction induced by the K+ [80 mM] and phenylephrine [1 microM] of aorta preparations. Data show that C. rotundus possess the spasmolytic, bronchodilator and vasodilator activities possibly through calcium channels blockade; validating its folkloric use in diarrhea, dyspepsia, bronchitis, asthma and hypertension in addition to antibacterial, antiemetic, antipyretic and analgesic activities